Figure 2
Chronological analysis of early immune process in reperfused kidneys. The P-selectin mRNA was studied as marker of endothelial activation (a). Innate immunity markers like Toll-like receptors 2 (TLR2) and TLR4 mRNA (b), MCP-1 protein (c), IL-1β and IL-6 cytokines mRNA (d) were investigated in kidney tissue. IL-1Rn and IL-10 cytokine mRNA expressions were also explored (e). The numbers of positively ED1 and CD3 stained cells per surface area (104/μm2) was counted on five different tissue sections for each experimental conditions (f). Shown are mean ± SEM. *p < 0.05 versus control. $ p < 0.05 between groups.