Figure 5

Frequency of CD4+granzyme+ and CD4+CD25+Foxp3+ T lymphocytes in mice treated with an anti-IL-10 mAb. FACS analyses of frequency of CD4+granzyme+ (A and C) and CD4+CD25+Foxp3+ (B and D) T lymphocytes were performed in mice untreated or treated with an anti-IL-10 mAb. The analyses were executed on TIL from treated (black columns) or untreated (white columns) mice. (A) and (B) panels refer to experiments performed in C57BL/6J mice challenged with B16F10 melanoma cells; (C) and (D) panels refer to experiments performed in Balb/c mice challenged with VAC ALCL cells. In all panels, data are expressed as percentage of positive cells out of the total CD3+ T cell population.