Figure 2

The effect of HPV 16 E2 on gC1qR expression levels in cervical squamous carcinoma cell lines (C33a and SiHa). Cells were treated with unmodified media (control), empty vector, HPV 16 E2 and HPV 16 E2 mutant for 48 h. A: Relative gC1qR gene expression levels are demonstrated in C33a and SiHa cells. gC1qR expression levels were analysed by real-time PCR. ***p < 0.001, **p < 0.01, ^#p > 0.05 versus the control group; ^▲▲▲p < 0.001, ^▲▲p < 0.01 versus the empty vector group; ^△△△p < 0.001, ^△△p < 0.01 versus the HPV 16 E2 group. B: gC1qR protein levels were measured in C33a and SiHa cells using Western blot analysis. The graph depicts relative gC1qR protein levels normalised to actin. The results are expressed as the means ± SD of three separate experiments. ***p < 0.001, ^#p > 0.05 versus the control group; ^▲▲▲p < 0.001 versus the empty vector group; ^△△△p < 0.001 versus the HPV 16 E2 group.