Figure 1
The effect of HPV 16 E2 on cervical squamous carcinoma cell line (C33a and SiHa) viability, migration and proliferation. Cells were treated with unmodified media (control), empty vector, HPV 16 E2 and HPV 16 E2 mutant for 48 h. A: C33a and SiHa cell viability was detected using a WST-1 assay. Sample absorbance was analysed using a bichromatic ELISA reader at 450 nm. **p < 0.01, *p < 0.05, #p > 0.05 versus the control group; ▲▲p < 0.01, ▲p < 0.05 versus the empty vector group. △△p < 0.01, △p < 0.05 versus the HPV 16 E2 group. B: The C33a and SiHa cell migration was measured with a transwell assay. Migrated cells were counted microscopically (400 X) in five different fields per filter. **p < 0.01, *p < 0.05, #p > 0.05 versus the control group; ▲▲p < 0.01, ▲p < 0.05 versus the empty vector group. △△△p < 0.001, △p < 0.05 versus the HPV 16 E2 group. C: C33a and SiHa cell proliferation. 3H-thymidine DNA incorporation results over 18 h of the final incubation. The results are expressed as the mean ± SD from 3 independent experiments. **p < 0.01, #p > 0.05 versus the control group; ▲▲p < 0.01, ▲p < 0.05 versus the empty vector group; △△p < 0.01 versus the HPV 16 E2 group.