Figure 3

Effect of SDF-1α preconditioning on IPCs under different glucose concentrations in vitro . a) The number of viable cells is plotted in relevance with normal BM-derived MSCs. There is a marked increase in cell survivability in pre-conditioned group than control and non pre-conditioned groups (n = 6). b) Cyto-protective effect of SDF-1α pre-conditioning on IPCs. Pre-conditioned IPCs showed low level of LDH compared to the control and non pre-conditioned groups indicating less cellular injury. c) Effect of SDF-1α pre-conditioning on cell proliferation under different glucose concentrations. Pre-conditioned IPCs showed higher rate of proliferation as compared to the control and non pre-conditioned groups. d) Glucose responsive insulin release by SDF-1α pre-conditioned IPCs. The IPCs were exposed to either 5.5 mM/L, 17 mM/L or 33 mM/L glucose. Insulin released from the IPCs upon glucose induction was determined by ELISA. The experiments were performed in triplicates and repeated three times. All values were expressed as mean ± SEM.