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Table 2 CTLA-4 expression in melanoma tissue microarray by immunohistochemistry

From: The engagement of CTLA-4 on primary melanoma cell lines induces antibody-dependent cellular cytotoxicity and TNF-α production

Melanoma cases CTLA-4 staining IRS
  % of positive cells Intensity  
1 10 3+ 3
2 70 2+ 6
3 10 1+ 1
4 30 1+ 2
5 5 1+ 1
6 80 2+ 6
7 70 2+ 6
8 70 2+ 6
9 80 2+ 6
10 0 0 0
11 85 3+ 9
12 0 0 0
13 10 1+ 1
14 0 0 0
15 0 0 0
16 0 0 0
17 5 1+ 1
18 5 1+ 1
19 0 0 0
20 60 2+ 6
21 90 2+ 6
22 70 2+ 6
23 30 1+ 2
24 0 0 0
25 100 1+ 1
26 0 0 0
27 80 3+ 9
28 95 3+ 9
  1. CTLA-4 expression by IHC staining of tissue microarrays consisting of 28 formalin-fixed, paraffin-embedded melanoma sections.
  2. IHC staining was performed with the murine anti-CTLA-4 14D3 mAb and detected with AP-conjugated secondary antibody and Fast Red chromogen.
  3. IRS: immunoreactive score (values from 0 to 9) for CTLA-4 expression was as follows: 0 (negative), 1–4 (low to intermediate), ≥6 (high). IRS score was obtained by multiplying the scores of % of positively stained cells and staining intensity. Scores for % of stained cells were: 0 (negative), 1 (1-29%), 2 (30-59%), 3 (60-100%). Scores for staining intensity were: 0 (negative), 1+ (weak), 2+ (moderate), 3+ (strong).