Melanoma cases | CTLA-4 staining | IRS |
|---|
| |
% of positive cells
|
Intensity
| |
|---|
1 | 10 | 3+ | 3 |
2 | 70 | 2+ | 6 |
3 | 10 | 1+ | 1 |
4 | 30 | 1+ | 2 |
5 | 5 | 1+ | 1 |
6 | 80 | 2+ | 6 |
7 | 70 | 2+ | 6 |
8 | 70 | 2+ | 6 |
9 | 80 | 2+ | 6 |
10 | 0 | 0 | 0 |
11 | 85 | 3+ | 9 |
12 | 0 | 0 | 0 |
13 | 10 | 1+ | 1 |
14 | 0 | 0 | 0 |
15 | 0 | 0 | 0 |
16 | 0 | 0 | 0 |
17 | 5 | 1+ | 1 |
18 | 5 | 1+ | 1 |
19 | 0 | 0 | 0 |
20 | 60 | 2+ | 6 |
21 | 90 | 2+ | 6 |
22 | 70 | 2+ | 6 |
23 | 30 | 1+ | 2 |
24 | 0 | 0 | 0 |
25 | 100 | 1+ | 1 |
26 | 0 | 0 | 0 |
27 | 80 | 3+ | 9 |
28 | 95 | 3+ | 9 |
- CTLA-4 expression by IHC staining of tissue microarrays consisting of 28 formalin-fixed, paraffin-embedded melanoma sections.
- IHC staining was performed with the murine anti-CTLA-4 14D3 mAb and detected with AP-conjugated secondary antibody and Fast Red chromogen.
- IRS: immunoreactive score (values from 0 to 9) for CTLA-4 expression was as follows: 0 (negative), 1–4 (low to intermediate), ≥6 (high). IRS score was obtained by multiplying the scores of % of positively stained cells and staining intensity. Scores for % of stained cells were: 0 (negative), 1 (1-29%), 2 (30-59%), 3 (60-100%). Scores for staining intensity were: 0 (negative), 1+ (weak), 2+ (moderate), 3+ (strong).
