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Table 1 Expression patterns of CTLA-4, MDA, SCA and NCA in 14 primary melanoma cell lines

From: The engagement of CTLA-4 on primary melanoma cell lines induces antibody-dependent cellular cytotoxicity and TNF-α production

Melanoma cell line CTLA-4 MDA SCA NCA
  Polyclonal antiserum Ipilimumab Melan A GP100 CD133 CD117 CD271 Nestin CD56
MECA 2.5 19.1 4.2 7.5 0.9 0.7 2.7 4.6 18.8
MECO 4.6 7.0 25.7 17.0 1.0 0.7 3.2 4.0 1.5
MEOL 3.4 3.7 10.9 2.5 1.1 0.6 26.8 6.1 24.5
MEMO 3.3 31.0 1.1 2.2 1.1 0.8 90.0 11.4 42.5
MEMR 3.5 4.3 2.4 2.1 0.9 0.9 1.0 1.9 32.0
MEPA 5.5 8.2 2.5 1.8 1.0 0.8 13.9 7.4 14.7
METR 6.9 3.8 18.8 23.6 10.3 0.8 12.5 2.3 1.4
MEBO 1.9 6.7 1.2 4.2 1.7 17.5 1.0 14.5 3.7
METU 3.5 7.0 1.0 1.0 1.3 2.0 22.5 9.5 12.0
MEMI 1.7 2.9 19.9 2.2 1.4 70.2 1.0 62.2 20.4
MEFE 2.0 7.2 22.8 1.0 1.0 1.6 18.3 2.2 46.9
MEPC 2.4 1.4 11.8 1.0 1.6 0.7 72.9 1.4 1.6
MECS 5.8 2.9 6.8 1.2 1.0 6.3 19.9 1.2 12.3
MEDB 3.7 1.7 0.8 2.7 1.0 8.5 4.8 1.9 2.6
  1. The 14 primary melanoma cell lines derived from metastatic lesions were stained with the indicated anti-CTLA-4 antibodies and analyzed by flow cytometry for the expression patterns of melanoma differentiation antigens (MDA), stem cell-related antigens (SCA) and neural crest antigens (NCA).
  2. Results were expressed as mean ratio of relative fluorescence intensity (MRFI), calculated as follows: mean fluorescence intensity (MFI) of CTLA-4 staining/MFI of irrelevant isotype-matched mAb staining.