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Figure 5 | Journal of Translational Medicine

Figure 5

From: Treatment of malignant effusion by oncolytic virotherapy in an experimental subcutaneous xenograft model of lung cancer

Figure 5

VACV treatment decreased tumor cell-produced hVEGF and infected TCCBVs in PC14PE6-RFP tumors. (A, B) PC14PE6-RFP tumor-bearing mice were either mock-infected or treated with 1 × 107 pfu GLV-1h68 (iv) 14 dpim. Tumor homogenates of mock- and GLV-1h68-treated tumors (7dpi, n = 6) were used for ELISA of human VEGF (A) and murine VEGF (B). Shown are the mean values +/− standard deviations. (C-F) Histological analysis of GLV-1h68-infected PC14PE6-RFP tumor sections 7 dpi revealed a GLV-1h68-infection of TCCBVs; PC14PE6-RFP tumor cells (red), GLV-1h68-infected cells express GFP (green), blood vessels were labeled with the CD31 antibody (blue), nuclei were labeled with Hoechst 33324 (white). All the different morphological formations of TCCBVs such as glomeruloid bodies (D) and garland-like vessels (E, F) were GFP-positive. (G) anti-VACV antibody labeling (blue) of tumor sections revealed VACV-positive labeling in GFP-positive areas. (H) Histological analysis of GLV-1h68-infected PC14PE6-RFP tumor sections revealed GLV-1h68-infection of invasive PC14PE6-RFP tumor cells at the tumor margin (arrowhead); PC14PE6-RFP tumor cells (red), GLV-1h68-infected cells (green), CD31-positive blood vessels (blue). All images are representative examples. Scale bars represent 300 μm (C, G), 75 μm (D-F), and 150 μm (H).

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