Figure 4

VACV infection-induced activation of the tumor vasculature. (A-D) PC14PE6-RFP tumor-bearing mice were either mock-infected or treated with 1 × 10⁷ pfu GLV-1h68 (iv) 14 dpim (n = 3). Seven dpi tumors were harvested and histological tissue sections were performed. The blood vessel density (A) was determined by counting the vessels/image in both groups and the mean fluorescence intensity of endothelial-associated CD31 was shown in (B). The content of either antigen-presenting MHCII-positive cells (monocytes, macrophages, dendritic cells, and B cells) (C) or Ly-6G-positive neutrophils (D) in mock- and GLV-1h68-treated tumors was determined by quantification of the mean fluorescence intensity of the antibody-labeled tumor sections.