Figure 4

(A) Alterations of miR-7 expression by depletion of E2 in T-47D. Levels of miR-7 expression were normalized using U6 probe. (B-D): Alterations of EGFR mRNA expression by depletion of E2 in breast carcinoma cells. Values for EGFR mRNA were depicted as percent expression to RPL13A by quantitative RT-PCR. (B) Expression of EGFR mRNA in ER-positive T-47D cells cultured in medium supplemented with 10% FBS or DCC-FBS. (C, D): Expression of EGFR mRNA in ER-negative SK-BR-3 (B) and MDA-MB-231 (C) cells cultured in medium supplemented with 10% FBS or DCC-FBS.