Figure 3

Immunohistochemical detection of CRKL protein in primary gastric cancer. TMA block sections were subjected to an immunohistochemical analysis using anti-CRKL monoclonal antibody (Y243; 1:100 dilution), Histofine Simple Stain Max-Po (Multi), and 3,3'-diaminobenzidine tetrahydrochloride. Intensity values of 0, 1, 2, and 3 are shown in (A), (B), (C), and (D), respectively. Bar = 50 μm. (E) Box-plot analysis of CRKL protein expression in gastric tissue. A statistically significant difference in the CRKL expression level was detected between non-cancerous gastric foveolar epithelium (n = 41) and gastric cancerous tissue (n = 360). (F) Representative result of the CRKL immunohistochemical analysis. A gastric cancer with a high CRKL expression level is shown. Bar = 500 μm. The inset is a magnified image. Bar = 50 μm. (G) Representative gastric cancer case showing both a high CRKL expression level and CRKL gene amplification. The high CRKL expression level (value = 2.6) was detected using an immunohistochemical analysis. Bar = 100 μm. The inset shows the amplification of CRKL (red) in the cancer cells. The CRKL signal (red) and the control signal for chromosome 22 (green) were detected using a FISH analysis. Nuclei are stained with DAPI.