Figure 4

Osteopontin enhanced FLJ10540-NPC transfectants to promote cell proliferation. (A and B, left panel) HA-tagged FLJ10540-stable clones of TW01 and Hone1 cells were established. The cell lysates were subjected to an immunoblot analysis with an anti-HA antibody. (A and B, right panel) Vehicle-TW01/-Hone1 and FLJ10540-TW01/-Hone1 stable transfectants with or without osteopontin (OPN) stimulation were seeded into 96-well plates with 1.0% of FBS. The cells were cultured for 1–3 days followed by MTT assay (OD570) to quantitate cell growth. The data were normalized against the OD570 value on day 1 of each treatment. The growth curve of TW01 and Hone1 cells are shown as the mean ±s.d. of three independent experiments.