Figure 6
Immunohistochemical staining of alpha-smooth muscle actin for quantification of small vessel (≤ 15.0 μm) (200x) in ischemic quadriceps at day 14 after CLI induction (n = 9). A to D) Significantly higher number of small vessels (≤ 15 μm) (red arrows) in normal control (C) group than in obesity (OB) and obesity control (OBC) groups, and notably higher number in OBC group than in OB group. * vs. other groups with different symbols, p < 0.01. Statistical analysis by ANOVA followed by Bonferroni multiple comparison post hoc test. Scale bars in right lower corner represent 50 μm. HPF = high-power field (200x).