Figure 4

Western blot results of oxidative stress and angiogenic factors by day 14 after CLI procedure (n = 9). A & B) Significantly higher oxidative index, protein carbonyls, in obesity animals than in normal and obesity controls, and significantly higher in obesity-control animals than in normal controls. * vs. other groups with different symbols, p < 0.005. (Note: Right lane and left lane shown on upper panel represent control oxidized molecular protein standard and protein molecular weight marker, respectively). DNP = 1-3 dinitrophenylhydrazone. C & D) protein expressions of stromal cell-derived factor (SDF)-1α and CXCR4 were significantly enhanced in obesity-control mice than in obesity mice in response to ischemic stress in ischemic muscle. * vs. other groups with different symbols, p < 0.02. Statistical analysis by ANOVA followed by Bonferroni multiple comparison post hoc test.