Figure 5
From: Molecular target based combinational therapeutic approaches in thyroid cancer
DIM targets VEGF secretion of thyroid cancer cells. B-CPAP and ML-1 thyroid cancer cells were seeded at a density of 5×105 cells per well in six-well culture dishes and allowed to adhere overnight. Thyroid cancer cells were then switched to serum free medium and incubated with ± 10-8 M E2 ± 10-6 M fulvestrant or left untreated for 24 hours. The conditioned medium was harvested and (A) ML-1 and (B) B-CPAP VEGF was assayed using VEGF ELISA kit. The asterisk denotes statistically significant differences (p < 0.05) between the treatment and untreated samples determined using a one way ANOVA test. The standard deviation represents n = 3 for each sample.