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Figure 2 | Journal of Translational Medicine

Figure 2

From: Molecular target based combinational therapeutic approaches in thyroid cancer

Figure 2

Effect of estrogen on the in vitro VEGF secretion by thyroid cancer cells. B-CPAP and ML-1 thyroid cancer cells were seeded at a density of 5X105 cells per well in six-well culture dishes and allowed to adhere overnight. Thyroid cancer cells were then switched to serum free medium and incubated with ± 10-8 M E2 ± 10-6 M fulvestrant or left untreated for 24 hours. The conditioned medium was harvested and (A) B-CPAP and (B) ML-1 conditioned medium was assayed for VEGF secretion using VEGF ELISA kit. The asterisk denotes statistically significant differences (p < 0.05) between experimental and untreated samples determined using a paired Student’s t - test. The standard deviation represents n = 3 for each sample.

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