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Figure 2 | Journal of Translational Medicine

Figure 2

From: Trehalose treatment suppresses inflammation, oxidative stress, and vasospasm induced by experimental subarachnoid hemorrhage

Figure 2

Effects of trehalose on the NF-кB pathway activated by hemolysate treatment. (A) Effect of trehalose on IкB-α phosphorylation and degradation in hemolysate-treated cells. RAW 264.7 cells were treated with 10% hemolysates or vehicle for 80 min. Cells were collected at the indicated time points, and then SDS samples were prepared. Amounts of phosphorylated IкB-α (P-IкB-α), total IкB-α (IкB-α), and β-actin were analyzed by western blotting. (B) The graph shows changes in the band intensities of total IкB-α, adjusted to the corresponding β-actin bands and calculated as intensity ratio relative to that of 0 min time-point, for 0, 20, 40, and 80 min. *P < 0.05 as compared to saline (Mann–Whitney‘s U test). n = 4 in each group. Whole experiments were repeated more than three times. All data are means ± SD.

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