Figure 1

Expression of pro-death and anti-death Bcl-2 genes in A375 melanoma xenografts. A375-RFP cells were isolated by laser microdissection (as indicated under Methods) 7, 14, and 21 days after tumor inoculation. The data, expressing fold change (quantitative RT-PCR, see under Methods for calculations), show mean values ± S.D. for 5-6 different experiments (*p < 0.05 for all genes displayed comparing A375-RFP cells isolated from in vivo growing tumors versus 24 h-cultured A375-RFP cells). No significant differences in expression of Bcl-2-related genes when control A375 and A375-RFP cells were compared in vitro(not shown).