A. TLR4 and MyD88 expression in EOC cell lines. Immunostaining for TLR4 protein in SKOV-3 (a) and A2780 (d) (×400). Immunostaining for MyD88 protein in SKOV-3 (b) and A2780 (e) (×400). Negative stainging for TLR4 and MyD88 using PBS (c, f). B. Expression of TLR4 and MyD88 in SKOV-3 and A2780 cell lines by using Western blot assays. C. The expression rates of MyD88 were gradually increased with the progress ovarian lesions. The positive rates of MyD88 in normal ovarian tissue, benign cysts, borderline tumors and EOC were 0, 33.3 %, 55.6 % and 77.1 %. D. (a) High expression of TLR4 was correlated with high expression of MyD88 in EOC. EOC with a high expression of TLR4 (N = 67) showed significantly high levels of MyD88 expression than did EOC with a low expression of TLR4 (N = 16) (P = 0.0045). (b) EOC with a high expression of MyD88 (N = 28) showed significantly high levels of TLR4 expression than did EOC with a low expression of MyD88 (N = 55) (P = 0.0117).