Figure 4

Cell proliferation of ESCC cell lines after overexpression or knockdown of Skp2. MTT assays were used to measure the optical density (OD) values of cells transfected by an empty pcDNA3.1 vector (Vec), pcDNA3.1-Skp2 plasmid (Skp2), scrambled siRNA as negative control (NC) or the siRNA specifically targeting Skp2 (siSkp2) at the indicated time points. (A) In KYSE30 cell line. For left panel, the asterisks represent as p = 0.004 (48 h), 0.001 (72 h), and 0.002 (96 h), respectively. For right panel, the asterisk represents as p = 0.001 (120 h). (B) In KYSE140 cell line. For left panel, the asterisk represents as p = 0.024 (48 h). For right panel, the asterisks represent as p = 0.016 (96 h) and 0.003 (120 h), respectively. (C) In KYSE180 cell line. For left panel, the asterisks represent as p = 0.042 (48 h), p < 0.001 (72 h), and p < 0.001 (96 h), respectively. For right panel, the asterisks represent as p = 0.004 (96 h) and p < 0.001 (120 h), respectively. Data are represented as mean +/- SD.