Figure 3
From: Skp2 expression unfavorably impacts survival in resectable esophageal squamous cell carcinoma
Transient overexpression or knockdown of Skp2 in ESCC cell lines. A western blot assay was used to detect the protein level of Skp2 36 h after transient transfection of an empty pcDNA3.1 vector (Vec), pcDNA3.1-Skp2 plasmid (Skp2), scrambled siRNA as negative control (NC) or the siRNA specifically targeting Skp2 (siSkp2). (A) KYSE30 cell line; (B) KYSE140 cell line; (C) KYSE180 cell line. The exogenous/endogenous Skp2 is indicated in the figure. β-Actin was used as a loading control.