Figure 6

H-HDL promotes pulmonary and hepatic metastasis of MDA-MB-231 and MCF7 cells in vivo. (a) MDA-MB-231 and MCF7 cells were pretreated with N-HDL and H-HDL for 24 hours then 4 × 10⁵ cells were intravenously injected into BALB/c nude mice via tail vein. After 20 days, lungs and livers were resected and analyzed for metastasis. Representative pictures of lungs and livers were shown. (b, c) Quantitative evaluation of detectable nodules on the surface of the whole lungs and livers was shown. Data is expressed as mean ± SEM. (*, P < 0.05; **, P < 0.01; ***, P < 0.001; one-way ANOVA). (d) Representative histological photomicrographs of lung and liver tissue sections stained with H&E (10×). Arrows indicate tumor islands. (e) MDA-MB-231 and MCF7 cells were pretreated with N-HDL and H-HDL for 24 hours then 2 × 10⁶ cells were injected into the mammary fat pad. BALB/c nude mice were received normal or oxidized HDL injection through vein tail every three days. After 40 days, lungs and livers were resected and analyzed for metastasis. Representative pictures of lungs and livers were shown. (f, g) Quantitative evaluation of detectable nodules on the surface of the whole lungs and livers was shown. Data is expressed as mean ± SEM. (*, P < 0.05; **, P < 0.01; one-way ANOVA). (h) Representative histological photomicrographs of lung and liver tissue sections stained with H&E (10×). Arrows indicate tumor islands.