Figure 4

H-HDL stimulates MDA-MB-231 and MCF7 cell migration, invasion and adhesion involving PKC pathway. (a) MDA-MB-231 and MCF7 cells were treated with N-HDL and H-HDL for 30 minutes at 100 μg/ml apoA-I, and then the cell lysates were subjected to the PepTag nonradioactive PKC assay. (b, c) MDA-MB-231 and MCF7 cells were preincubated with 5 nM staurosporine, a PKC inhibitor along with N-HDL and H-HDL at 100 μg/ml apoA-I respectively for indicated hours (6 hours for MDA-MB-231 and 18 hours for MCF7 in transwell migration assay; 18 hours for MDA-MB-231 and 24 hours for MCF7 in transwell invasion assay). Migrated cells (b) and invasive cells (c) were counted and the results were expressed in comparison to control. (***, P < 0.001; student's t-test).(d, e) MDA-MB-231 and MCF7 cells were pretreated with 5 nM staurosporine, a PKC inhibitor, for 3 hours and then along with N-HDL and H-HDL at 100 μg/ml apoA-I respectively for another 6 hours. Cell adhesion to HUVEC (d) and attachment to ECM (e) were measured. (***, P < 0.001; student's t-test).