Figure 3

Intergrins are involved in the increased invasion and adhesion of breast cancer cells induced by H-HDL.MDA-MB-231 and MCF7 cells were treated with N-HDL and H-HDL at 100 μg/ml apoA-I for 6 hours. Integrin β1 (a), integrin β2 (b), integrin β3 (c) and integrin αv (d) levels on the cell surface were measured by cell ELISA with five parallel wells. Results were expressed as percentage of HDL treated cells in comparison to control and data is expressed as mean ± SEM. (*, P < 0.05; **, P < 0.01; ***, P < 0.001 by one-way ANOVA). (e) MDA-MB-231 cells were incubated with N-HDL and H-HDL at 100 μg/ml apoA-I along with no antibody, negative control antibody, or anti-integrin (β1, β2, β3, αv) antibodies respectively in upper chamber for 18 hours. (***, P < 0.001; one-way ANOVA). MDA-MB-231 cells were pretreated with N-HDL and H-HDL at 100 μg/ml apoA-I for 6 hours and then incubated with no antibody, negative control antibody, or anti-integrin (β1, β2, β3, αv) antibodies for 30 minutes at 37°C before the adhesion to HUVEC (f) and attachment to ECM (g). Antibody-blocked groups were compared with control (antibody-untreated groups). Data is expressed as mean ± SEM with five parallel wells. (**, P < 0.01; ***, P < 0.001; one-way ANOVA).