Figure 2

H-HDL promotes more MDA-MB-231 and MCF7 cell adhesion to HUVEC and attachment to ECM. (a) MDA-MB-231 and MCF7 cells were pretreated with N-HDL and H-HDL at 100 μg/ml apoA-I for 6 hours and then seeded onto HUVEC-coated 96-well plate for 30 minutes. Relative cell adhesion was determined by Rose Bengal assay and expressed as percentage of HDL treated cells in comparison to control. Data is expressed as mean ± SEM with five parallel wells. (**, P < 0.01; ***, P < 0.001; one-way ANOVA). (b) MDA-MB-231 and MCF7 cells were pretreated with N-HDL and H-HDL at 100 μg/ml apoA-I for 6 hours and then plated onto matrigel-coated 96-well plate for 30 minutes. Relative cell adhesion was determined by MTT assay and expressed as percentage of HDL treated cells in comparison to control. Data is expressed as mean ± SEM with four parallel wells. (*, P < 0.05; ***, P < 0.001; one-way ANOVA).