Figure 1

H-HDL is more effective in stimulating MDA-MB-231 and MCF7 cell proliferation, migration and invasion. MDA-MB-231 (a) and MCF7 (b) were treated with N-HDL and H-HDL respectively for 12, 24, 48, 72 hours at 100 μg/ml apoA-I. Relative cell proliferation was measured by MTT assay and expressed as percentage of HDL treated cells in comparison to control. (*, P < 0.05; **, P < 0.01; ***, P < 0.001; one-way ANOVA). (c) MDA-MB-231 and MCF7 cells were incubated with N-HDL and H-HDL respectively at 100 μg/ml apoA-I in upper chamber for indicated time. Transwell assay was used to measure cell migration and pictures were shown in high power (100×). (d) Migrated cells were counted and the results were expressed as percentage of HDL treated cells in comparison to control. (***, P < 0.001; one-way ANOVA). (e) Monolayer MDA-MB-231 and MCF7 cells were wounded by manual scraping and incubated with N-HDL and H-HDL respectively at 100 μg/ml apoA-I for indicated time and then photographed in high power (100×). (f) Cells migrated into the gaps were counted and the results were expressed as percentage of HDL treated cells in comparison to control. (***, P < 0.001; one-way ANOVA). (g) MDA-MB-231 and MCF7 cells were treated with N-HDL and H-HDL respectively in upper chamber for indicated time at 100 μg/ml apoA-I. Transwell assay was used to measure cell invasion and pictures were shown in high power (100×). (h) Invasive cells were counted and the results were expressed as percentage of HDL treated cells in comparison to control. (**, P < 0.01; ***, P < 0.001; one-way ANOVA).