Figure 1From: Responses of human adipose-derived mesenchymal stem cells to chemical microenvironment of the intervertebral discCharacterization of isolated human ADMSCs by osteogenic and adipogenic differentiation. A: Human ADMSCs were expanded in monolayer culture (×40). B: ADMSCs were cultured in osteogenic media for 21 d and stained positive with alizarin red. Red staining marked mineral deposition in their newly formed extracellular matrix (×100). C: ADMSCs were cultured in adipogenic media for 14 d and stained positive with Oil red O staining. The lipid drop appeared red under fluorescence microscope (×100).Back to article page