Figure 5
Effect of prostaglandin treatment on expression of gap junction proteins-connexins 43, 30 and 26. A: Representative immunoblots of connexins 43, 30 and 26 in untreated (M-) and Misoprostol treated (M+) cervix in first trimester. C-positive control. B: Graphical representation comparing expressions of connexin- 43 (CX-43), connexin-30 (CX-30) and connexin-26 (CX-26) in Misoprostol treated (M+, n = 10) versus untreated (M-, n = 5) cervix on Western blotting. The expression of the studied connexions did not differ between Misoprostol-treated and untreated tissue. C and D: Representative confocal photomicrographs depicting no change in localisation patterns of connexins 30 and 26 in untreated (M-) (Figure 5C) versus Misoprostol treated (M+) (Figure 5D) cervix. Bottom row showing positive controls (myometrium) and negative controls (cervical tissue) for connexins 26 and 30. DAPI- nuclear staining. e- epithelium, s-stroma. Arrows at basement membranes. Ă—20 air objective, NA 0.8, Bar = 8 ÎĽm.