Morphology, growth pattern, and expression of β1-integrin. (A) Differential interface contrast (DIC) images of 3D multicellular morphologies from structures formed by epithelial endometrial cancer cell lines after 5 days cultured on poly-HEMA coated cultureware. Ishikawa cell line formed tightly compact spheroids, which were resistant to trypsin-EDTA digestion. RL95-2 and KLE cell lines formed cell aggregates and clusters respectively, and they were easily dissociated by trypsin-EDTA treatment. The scale bar is 100 μm. (B) The growth of Ishikawa, RL95-2 and KLE cell lines cultured in 3D structures. The total cell number was counted by a haemocytometer at intervals of 2 days. The open circle (○), dark circle (●) and triangle (▼) represent Ishikawa, RL95-2 and KLE cell lines, respectively. (C) The expression of β1-integrin was observed in Ishikawa, RL95-2 and KLE cell lines.