Figure 2

Phenotypic and functional analysis of differently matured DC. (A) Percent of CD83-positive cells measured on 7-day, 4C-matured DC (n = 6) as well as 3-day, 4C- and 5C+R848-matured DC (n = 4). Percent of CD14-positive cells ranged between 0.9 and 5.7 percent in the tested populations. (B) Ratio of secreted IL-12(p70) to IL-10 assessed by standard ELISA of signal-3-assay supernatants. Analyzed DC were matured using the 7-day, 4C protocol (n = 7) or the 3-day protocol in combination with 4C or 5C+R848 mediated maturation (n = 4). (C) Ratio of the mean fluorescence intensity of CD80 to CD274 (B7-H1) assessed on 7-day, 4 C generated mDC (n = 3) as well as 3-day DC matured with 4C or 5C+R848 cocktails (n = 6). Significance was ascertained using a two-tailed Mann-Whitney U test and p-values were defined as following: ** = p < 0.005; * = p < 0.05; n.s. = p > 0.05.