Figure 1

Expression of SPARC in cell lines. (A) Quantitative RT-PCR detection showed that NPC cell lines of CNE1, CNE2, HONE1, SUNE1 and C666 presented higher level of mRNA expression of SPARC than that in NPEC2 Bmi-1. (B) Western blot analysis showed that the expression of SPARC protein levels in NPC cell lines was higher than that in NPEC2 Bmi-1. (C) Quantitative RT-PCR detection showed that 5-8 F presented higher level of mRNA expression of SPARC than that in 6-10B (P = 0.002). (D) Western blot analysis showed that the expression of SPARC protein levels in 5-8 F was higher than that in 6-10B (P = 0.001). Results are shown as expression relative to GAPDH and are means (± SD) of 3 experiments.