Figure 6

Histological analysis in C666-1 xenografts. C666-1 xenografts were resected at the end point of experiments, fixed and embedded in paraffin. Sections (5 μM thick) were mounted on Poly-L-Lysine treated slides. Upper, Ki67 expression assay. Sections were microwaved for 10 min for “antigen retrieval” and incubated with Ki67 antibody (1:200), followed by visualization with DAB using a Streptavidin-Horseradish Peroxidase kit. Lower, TUNEL labeling for apoptosis analysis. TUNEL labeling was performed with a fluorescent in situ cell death detection kit, according to the manufacturer’s instruction (Roche Applied Science, Germany).