Figure 4

Ad-IFNγ effectively expressed human IFN-γ in NPC xenografts in nude mice. Mice were intratumorally injected with 2×10⁹ pfu of Ad-IFNγ, and then collected tumor tissues (A) and bloods (B) 1 day, 3, 5 and 7 days after Ad-IFNγ injection (mice before injection marked “day 0” as negative control, 3-4 mice per time point). Tumor tissues were made into homogenates in cold PBS, and then human IFNγ concentrations in the tissue homogenates and blood plasmas were measured by using human interferon-γ ELISA kit (Boster, Wuhan, China).