Figure 5

Viability, migration and apoptosis of C33a and SiHa cells. A, a: C33a and SiHa cell viability was detected via a WST-1 assay. Sample absorbance was analysed using a bichromatic ELISA reader at 450 nm; b: The migration of cells was measured by Transwell assay. The migrated cells were counted microscopically (400 X) in five different fields per filter; c: Numbers of apoptotic cells were measured by flow cytometric analysis. These results are shown as the means ± SD from 3 independent experiments. *p < 0.05, **p < 0.01 versus p53 (-), gC1qR vector (+) and empty vector (-) group; ^Δp < 0.05, ^δδp < 0.01, ^#p > 0.05 versus p53 (-), gC1qR vector (-) and empty vector (+) group; ^★★p < 0.01 versus p53 wt (+), gC1qR vector (-) and empty vector (+) group; ^▲p > 0.05 versus p53 mt (+), gC1qR vector (-) and empty vector (+) group. B, a: C33a and SiHa cell viability was detected via a WST-1 assay; b: The migration of cells was measured by Transwell assay; c: Numbers of apoptotic cells were measured by flow cytometric analysis. These results are shown as the means ± SD from 3 independent experiments. ***p < 0.001, **p < 0.01, ^#p > 0.05 versus gC1qR vector (+) group; ^▲p > 0.05 versus empty vector (+) group; ^★★p < 0.01, ^★★★p < 0.001 versus empty vector (+) group; ^Δp > 0.05 versus empty vector (+) and p53 siRNA (+) group.