The biological effects of gC1qR overexpression on C33a and SiHa cells. A, a: The expression levels of gC1qR mRNA were analysed by real-time PCR. b: The levels of gC1qR protein were measured by Western blot analysis. The results are shown the means ± SD of three separate experiments. **p < 0.01 compared with the empty vector group. B, a: ROS generation was quantified by measuring the fluorescence of H2DCFDA for 30 min with fluorescence microscopy. b: Intracellular Ca2+ levels were monitored using the fluorescence probe fluo-4 AM. c: Time-dependent changes in relative Δψm values were measured by the fluorescence of JC-1(590 nm: 527 nm). These data are representative of three separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001, #p > 0.05 versus the empty vector group. C, a: The viability of cells was detected via a WST-1 assay. Sample absorbance was analysed using a bichromatic ELISA reader at 450 nm; b: The migration of cells was measured by a Transwell assay. The migrated cells were counted microscopically (400 X) in five different fields per filter. #p > 0.05, *p < 0.05, **p < 0.01 versus the empty vector group; c: Apoptotic death of C33a and SiHa cells was assessed by flow cytometric analysis. D: The level of p53 was measured by Western blot. The results are expressed as the means ± SD of three separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001, #p > 0.05 versus the empty vector group.