Skip to main content
Figure 4 | Journal of Translational Medicine

Figure 4

From: NF-κB is activated in response to temozolomide in an AKT-dependent manner and confers protection against the growth suppressive effect of the drug

Figure 4

AKT is involved in TMZ-induced activation of NF-κB. (A) The cells were transiently transfected with 50 nM siAKT1 or scrAKT (Scr) and 24 h later exposed to 50 μM TMZ+10 μM BG, or to BG alone. After 72 h of culture, total cell extracts were analyzed for AKT, IκB-α and NF-κB2/p52 expression by immunoblotting (see legend of Figure 2). The results are representative of two independent experiments. (B) The cells were transiently co-transfected with the pNF-κB-Luc and pRL-null vectors and 24 h later incubated with 50 μM TMZ+10 μM BG or with BG alone. Luciferase assays were performed after 72 h of drug exposure. Data are expressed in terms of fold change (see legend of Figure 1). Values represent the mean of four independent experiments performed with duplicate samples. Bars, SEM. *p<0.05 according to paired Student’s t test analysis performed comparing firefly luciferase activity/μg protein detected in TMZ-treated pUSE2 cells with that detected in the corresponding control cells. The difference between firefly luciferase activity/μg protein of TMZ-treated KD12 cells and that detected in the corresponding control cells was not statistically significant. (C) The cells were treated with 50 μM TMZ+10 μM BG or with BG alone for 72 h. Nuclear extracts were then examined for the expression of RelA and NF-κB2/p52 by immunoblotting (see legend of Figure 2). The results are representative of two independent experiments.

Back to article page