Figure 5
Intracellular localization of CXCR4 and CXCR7 in SLE B cells. (A) Total pools of CXCR4 and CXCR7 were simultaneously detected by flow-cytometric analysis in CD19+-gated PBMC from healthy and SLE subjects. Staining was performed on fixed and permeabilized leukocytes with both 9C4 (PE) and 12G5 (APC) mAbs. Quadrants were set on controls stained with the corresponding isotype control. Representative dot-plots demonstrating coexpression of CXCR4 and CXCR7 in total B cells from a healthy individual and two patients with either inactive or active SLE are shown. (B) The proportions (mean ± SEM) of total (CD19+) B cells, naive (CD19+CD27-) B cells, memory (CD19+CD27+) B cells and PC (CD19lowCD27high) expressing CXCR4 and/or CXCR7 in healthy and SLE subjects are given. *P < .05, **P < .005 and ***P < .0005 compared with control B cells.