Figure 2

Modulation of EGFR and p-EGFR expression in ESCC cells by Nimotuzumab. Both KYSE30 and TE-1 cell lines were cultured with 0, 100 and 200 μg/mL Nimotuzumab for 72 hours in medium containing 10% FBS. Western blot analysis was performed to examine the expression levels of EGFR and p-EGFR. Nimotuzumab did not influence the levels of EGFR in both cells, but it down-regulated the expression of p-EGFR in KYSE30 cells with overexpression of EGFR. Representative result was shown of triplicate experiments.