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Figure 1 | Journal of Translational Medicine

Figure 1

From: Nimotuzumab promotes radiosensitivity of EGFR-overexpression esophageal squamous cell carcinoma cells by upregulating IGFBP-3

Figure 1

Nimotuzumab enhanced the sensitivities of EGFR overexpressed ESCC cells to RT in vitro.(a and b) KYSE30 and TE-1 cells were incubated with or without Nimotuzumab (100 μg/mL) for 24 hours, and exposed to the indicated doses of RT. The colonies were stained and counted, and survival curves were constructed from three independent experiments. N = 3, bars, ± SD. The result is representative at 6 Gy of RT dosage. (c) KYSE30 and TE-1 cells were treated with RT (2 Gy, 10 Gy) in presence or absence of Nimotuzumab (100 μg/mL) for 48 hours. The control, RT and combination treatment groups were collected for staining with annexin-V/PI. The samples with a statistically significant difference compared with the groups as indicated (N = 3, bars, ± SD.) (d) Cell lysates were subjected to immunoblot with caspase-3-specific antibody for cleavage of caspase-3.

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