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Figure 7 | Journal of Translational Medicine

Figure 7

From: Indoleamine 2,3-dioxygenase 1 (IDO1) activity correlates with immune system abnormalities in multiple myeloma

Figure 7

STAT3 signaling and expression of TDO by MM cell lines. Panel A: U266 and MOLP-8 MM cells were pre-treated with WP1066, a STAT3 inhibitor, and then activated with IFN-γ for 72h. Cell lysates were immunoblotted with phosphorylation-specific antibodies against STAT3 and with anti-human IDO antibodies. Densitometry is indicated above each lane, relative to Hsp-70. Panel B: The expression of TDO protein was measured in U266 and MOLP-8 MM cells, relative to U87 glioma cells and primary rat hepatocytes, used as positive control. Densitometry is indicated above each lane, relative to Hsp-70. Panel C: Tryptophan and KYN concentrations were measured in supernatants of MOLP-8 MM cells that were activated with 100 IU IFN-γ in the presence of either 1MT or 680C91, a selective TDO inhibitor (TDOi). Results are representative of 4 independent experiments run in duplicate.

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