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Figure 1 | Journal of Translational Medicine

Figure 1

From: Indoleamine 2,3-dioxygenase 1 (IDO1) activity correlates with immune system abnormalities in multiple myeloma

Figure 1

IDO1 activity in patients with MM. Panel A: KYN and tryptophan levels were measured with RP-HPLC both in patients with MM and in age-matched healthy controls (ctrls). Bars depict median values and inter-quartile ranges. Statistical comparisons were performed with the Kruskal-Wallis test for unpaired determinations with Dunns correction for multiple comparisons. Panel B: IDO protein was detected by Western blotting in malignant PC that were magnetically isolated with CD138 microbeads from the BM of 17 MM patients. The U937 monocytic leukemia cell line and U266 MM cell line were used as negative and positive control for IDO expression, respectively. Densitometry is indicated above each lane, relative to GAPDH. Panel C: BM mononuclear cells from 5 randomly selected MM patients were stained with an anti-human IDO mAb and then analyzed by flow cytometry. In this representative experiment, BM-resident T cells stained negatively for IDO, at variance with BM CD138+ PC. Markers were set according to the proper isotypic control.

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