Figure 1
The expression pattern of Rab27B in BC tissues. (A) Western blot analysis of Rab27B protein expression in 8 representative paired BCs (T) and NATs (N). Equal loading of protein is shown by α-tubulin. (B) Rab27B mRNA and protein expression in human BCs (T) and NATs (N) by Real-time PCR (left panel) and IHC analysis (right panel). Rab27B mRNA and protein expression in 12 primary BCs is upregulated compared to their NATs. (C) Negative staining of Rab27B in normal breast tissue. (D) Weak staining of Rab27B in FA. (E) Moderate staining of Rab27B in DCIS. (F) BC cells showing strong staining of Rab27B. (G) Strong Rab27B staining in metastatic lymph nodes. (H) Statistical analysis revealed a significantly ascending pattern of Rab27B expression ranging from NAT to BC to distant metastases nodes.