Table 2 Comparison of proposed QL-MI assay with hs-ELISA and nephelometry
QL-MI (One-step format) | QL-MI (Two-step format) | Nephelometrya | ||
|---|---|---|---|---|
Sensitivity | 0.21 μg/L | 0.06 μg/L | 0.039 μg/L | 1000 μg/L |
Intra-day precision(CV) | < 2.17% | < 1.75% | 2.4-10.4% | 2.6-11.1% |
Inter-day precision(CV) | < 9.52% | < 2.27% | 1.7-17.6% | 4-12.1% |
Linearity | 0.001-100 mg/L | 0.001-100 mg/L | 10-5-0.02 mg/L | 1-960 mg/L |
Detection time | 1 hour | 1.5 hours | 2-6 hours | 40 minutes |