Figure 4
Cross-reaction of the two assay formats; 100 μL of a 10 mg/L solution of albumin (red column), hemoglobin (green column), and immunoglobulin G (blue column) were introduced into the coating antibody-immobilized microplate to perform the cross-reaction. No significant differences were observed between the fluorescence intensities induced by albumin, hemoglobin, and immunoglobulin G. However, the fluorescence intensities induced by nonspecific binding were significantly lower than those induced by the specific hs-CRP antigen in the one-step assay (p < 0.05) and in the two-step assay (p < 0.05). Error bars indicate the standard errors of 3 independent experiments.