Figure 1

Scheme for the two formats of QL-MI. These assays share the same procedure at the first two steps, including monoclonal antibody coating and antigen introduction. The difference is that A), the QD-mAb conjugate was introduced into the microplate prior to fluorescence detection in the one-step format while B), the QD-streptavidin was added 20 min later than the biotinylated secondary antibody in two-step format.