Figure 3

mRNA levels of acta 1 and slc38a2 in confluent L6 cells refed low (0.28 mM) or high (9 mM) total amino amino acid concentrations compared to cells cultured for 24 hours in starvation medium (0,14 mM) as described in Materials and methods . Acta 1 was significantly increased in low and high AA refed cells at 60 min compared to 24 hours starved cells (p<0.01). Slc38a2 concentrations were decreased at 60 min (p<0.05, n=9).