Figure 2

mRNA levels of Myosin heavy chain 2A, ACTA 1 and SLC38A2 in three different models with increased protein synthesis and translation initiation at amino acid provision . Rectus abdominus skeletal muscle tissue from surgical patients who received total parenteral nutrition compared to saline infusions for 12 hours; Mice “refed” with standard chow for 3 hours after 12 hours of starvation and L6 skeletal muscle cells cultured in presence of either low (0,28mM) or high (9 mM) total amino acid concentrations for 18 hours. Muscle MHC2A transcript levels were significantly decreased in parenterally fed patients (n=5) compared to saline infused control patients (n=5) (* p<0.05) while Mhc 2A /acta 1 transcrips were not different in mixed hind limb muscle tissue from starved/refed mice (Acta 1 p<0.10, MCH2A p<0.18, n=16) or cultured L6 cells (p<0.3). Slc38a2 (Snat2) levels were significantly decreased in L6 cells cultured in presence of high amino acid concentrations (p<0.01).