Figure 3

Increased TAEC interaction with tumor cells and up-regulated expression of E-selectin, ICAM-1 and VCAM-1 after insufficient RFA. (A-B) TAECs were cultured after insufficient RFA, and HepG2-GFP cells were added after 24, 48 and 72 h. Representative micrographs of TAECs regarding the interaction with HepG2-GFP cells are shown. Columns: means from three individual experiments with five samples per group; bars: SE; ***: P <0.001. (C) The expression of E-selectin, ICAM-1 and VCAM-1 on the surface of TAECs after insufficient RFA was detected using cell ELISA analysis. Columns: means from three individual experiments with five samples per group; bars: SE; *: P <0.05; **: P <0.01; ns: no significance. (D) Expression of E-selectin, ICAM-1 and VCAM-1 in TAECs after insufficient RFA detected by western blot analysis. Data are the representative results of three independent experiments with five samples per group.