Verification of TAECs from HCC. (A) Representative immunofluorescence analysis of TAECs showing positive expression of the endothelial markers CD31, CD34, VEGFR1, VEGFR2 and vWF, and negative expression of the macrophages and fibroblast markers CD68 and α-SMA. (B) Western blot analysis used to verify the immunofluorescence results and assess the purity of the isolated CD31+ TAECs. (C) Micrograph representative of the uptake of Dil-Ac-LDL in TAECs. Five TAECs preparations were analyzed with similar results.