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Table 3 Number of cells harvested, number of population doublings and doubling time for each BMSC passage

From: The establishment of a bank of stored clinical bone marrow stromal cell products

   T-75 flasks 2-layer cells factories First culture in 10-layer cell factories Second culture in 10-layer cell factories   
Donation Number Donor CFE Yield (×106) Yield (×106) Population Doublings Doubling Time (hr) Yield (×109) Population Doublings Doubling Time (hr) Yield (×109) Population Doublings Doubling Time (hr) Cumulative Population Doublings Units in Storage
W092110086001 1 6 6.5 94.4 3.9 30.9 0.81 3.2 37.3 1.73 3.4 34.9 10.5 10
W092110086002 2 18 30.3 372 4.5 26.4 0.99 3.6 33 2.43 3.9 30.6 12 19
W092110086003 3 9 21 314.3 4.3 27.9 1.28 4 30 1.61 3.3 36 11.6 14
W092110086004 4 0 0.2 NA NA NA NA NA NA NA NA NA NA NA
W092110086005 5 11.5 52.1 456.4 4.8 24.8 1.23 3.9 30.4 4.76 4.9 24.5 13.6 40
W092110086006 7 15 28 372.5 4.5 26.4 0.9 3.5 34.3 2.17 3.8 31.9 11.8 20
W092110086007 10 1.5 25.4 255.4 4 30 1.1 3.8 31.8 1.66 3.4 35.6 11.2 15
W092111086001 11 7.5 3.9 27.9 3.9 30.9 0.15 2.9 49.8 0.99 3 47.2 8.8 9
W092111086002 1 7 3.1 NA NA NA NA NA NA NA NA NA NA NA
W092111086003 14 5.5 12.9 233.2 4.3 28 0.97 3.6 33.3 2.52 3.98 30.2 11.9 23
W092111086004 15 4.5 2.2 NA NA NA NA NA NA NA NA NA NA NA
  1. NA not applicable; CFE colony formation efficiency